• Discussion Fanatics

    However, once you use the 100x objective lens, the light refraction when using a dry lens is noticeable. By placing a substance such as immersion oil with a refractive index equal to that of the glass slide in the space filled with air, more light is directed through the objective and a clearer image is observed.

  • Discussion Fanatics

    Oil immersion is the technique of using a drop of oil to wet the top of the specimen or slide cover and the front of the objective lens. This effectively immerses or bathes the light path between the lens and object viewed, allowing finer details to be seen.

    Also Know, when and why should you use immersion oil? When to use an oil immersion lens Use an oil immersion lens when you have a fixed (dead - not moving) specimen that is no thicker than a few micrometers. Even then, use it only when the structures you wish to view are quite small - one or two micrometers in dimension.

    Also asked, why must oil be used to obtain the best resolution with a 100x lens?

    Oil is used to displace air between the lens and specimen. Bending of light rays. Occurs when light passes from a medium of one refractive index to another.

    Does immersion oil increase magnification?

    Inmersion oil does not increase magnification. High magnifications come with high numerical appertures (although one can have a low-magnification high-numerical apperture objective). High numerical appertures are required to capture more light and improve resolution.

What is discussplaces?

DiscussPlaces is a place to make new friends and share your passions and interests. Quench your thirst for knowledge, discuss places with other aficionados, and swap recommendations. Are you an aspiring foodie who dreams of living in New York? Or perhaps you are looking for the best chicken wings in Cincinnati? Then this is the place for you! Any one can join in with a passion or interest – whether it be talking about their favorite restaurant in Barcelona or raving about their latest trip to Italy. Join us!



This page shows discussions around "Why is immersion oil used with the 100x objective lens?"

Tags

medical health eye and vision conditions oil immersion oil lens immersion light

Add a quick comment about your valuable suggestion to help us improve.




We are glad to make you happy, kindly share this feedback with your friends and family to know as well



Where is it?